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KMID : 0377519910160030299
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Chung-Ang Journal of Medicine
1991 Volume.16 No. 3 p.299 ~ p.313
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A Study on the Change of Xanthine Oxidase Activities in Experimental Cerebral Infarction
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Abstract
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To oberve the activity of the xanthine oxidase in cerebral ischernia, an ischemic model was wade using a transorbital occlusion of the middle cerebral artery(MCA) in cats. Xanthine oxidase activity was measured by means of high performance liquid chromatography with electrochemical detection of uric acid.
The results were as follows.
1. The infarction in the territory of the left middle cerebral artery(MCA) was identified with in tracardiac perfusion of a 2% triphenyltetrazolium chloride(TTC) solution after transorbital occlusion.
2. There was no significant difference between the control and ischemia groups in the total xanthine oxidase activity.
3. In control group, 38.2% of the total xanthine oxidase was in the oxygen-dependent superoxide-producing oxidase group(Type 0). It had been increased with time in ischemia, despite the total xanthine oxidase activity remaining the same; particularly at 12 hours(p< 0.05) and 24 hours (p< 0.01) after occlusion of the left MCA.
4. In the healthy side, the activity of type 0 and its ratio to the total xanthine oxidase activity has not been increased.
5. In the ischemic side, the conversion of type D xanthine oxidase to the type 0 xanthine- oxidase was demonstrated and its rate had been increased with the passage of time.
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